Broadening of Neutralization Activity to Directly Block a Dominant Antibody-Driven SARS-Coronavirus Evolution Pathway (original) (raw)
Figure 1
Serologic analysis.
A, 11 of convalescent serum samples were obtained from SARS patients of the 2002/03 outbreak in China. Neutralizing activities of these serum samples were analyzed against pseudotyped viruses bearing the S protein of Tor2 or GD03 at indicated dilutions. Data were shown in a box and whiskers graph. The box extends from 25th percentile to the 75th percentile, with a line at the median. The whiskers above and below the box indicate the 95th and 5th percentiles. The dots above and below the box showed highest and lowest data points. A symbol of “*” indicates the data points of a SARS-CoV negative healthy human serum sample. IC90 for each patient serum was calculated and the statistic analysis was done with IC90 value using one way ANOVA for correlated samples. The same data presentation and the statistic analysis methods were used for the following panels B and D. B, Neutralization assay of Tor2 and GD03 pseudotyped viruses with six civet serum samples collected from animal market in Guangzhou in Jan. 2004. Similarly as Fig. 1A, “*” indicates the data points for a SARS-CoV negative civet cat serum sample. C, Neutralization assay of Tor2 and GD03 pseudotyped viruses with four serum samples from 2003/04 outbreak. The samples labeled in the graph as patient 1–4 that were the same patients described before [5]. D. Ten serum samples from civet cat farmers collected in June 2003in Guangdong Province were analyzed against Tor2 and GD03 pseudotyped viruses. E, Tor2 -RBD binding activity of 2002/03, 2003/04 outbreak, and Civet cat farmers' serum samples were analyzed by ELISA. These serum samples were the same as those used in Panel A, C, and D, respectively. Dilution of serum was 1∶180. The data was presented the same way as panel A and unpaired Student t-test was used for statistical analysis. The average and standard deviation (SD) of background binding of two SARS-CoV negative human serum samples (Ctrl.HS) were also shown. F, A comparison of the competition ability of different serum samples for 80R's binding to Tor2-RBD were evaluated by ELISA. Dilution for all samples was 1∶20. The non-specific competition for 80R's binding to Tor-RBD by two Ctrl. HS or one control civet cat serum (Ctrl.CS) was also shown. Serum samples were corresponding to those used in panel A, B, C or D. The data presentation and statistic analysis were done the same way as panel E.