Extracellular DNA Chelates Cations and Induces Antibiotic Resistance in Pseudomonas aeruginosa Biofilms (original) (raw)
Figure 4
Extracellular DNA induces PA3553 gene expression in planktonic cultures.
Effects of (A) salmon sperm DNA, (B) salmon sperm DNA in the presence of excess (5 mM) Mg2+ (C) sonicated P. aeruginosa PAO1 genomic DNA and (D) DNAsed salmon sperm DNA on the expression of the PA3553::lux transcriptional fusion in planktonic cultures. Gene expression was normalized to growth for each condition and CPS/OD600 values are presented. Each growth experiment was performed at least five times and representative curves are shown. (D) Buffer control indicates that this sample was treated identically to the DNAsed DNA sample except for the addition of DNAseI enzyme. Standard deviations for each experiment were not greater than +/−10% of the value shown.