An RIG-I-Like RNA Helicase Mediates Antiviral RNAi Downstream of Viral siRNA Biogenesis in Caenorhabditis elegans (original) (raw)
Figure 1
Screening for viral immunity genes in C. elegans by feeding RNAi.
(A) Genome structure and expression of wildtype FHV RNA1 (FR1) and its B2-deficient mutant, FR1gfp, that expresses the enhanced GFP in place of B2. (B) Detection of green fluorescence in FR1gfp reporter worms after feeding RNAi targeting specific genes or the commonly used L4440 vector as indicated, photographed 48 hours after induction of the replicon replication. (C) Accumulation of FR1gfp genomic (RNA1) and subgenomic RNA (RNA3) by northern blotting in FR1gf worms with (lanes 3–12) and without (lanes 1–2) feeding RNAi of specific worm genes. Two independent tests were analyzed for each E. coli strain. Methylene blue staining of total RNA was provided to show equal loading.