Quantitative Metabolomics Reveals an Epigenetic Blueprint for Iron Acquisition in Uropathogenic Escherichia coli (original) (raw)
Figure 3
Siderophore production by the model uropathogen UTI89 and selected mutants.
A) MS/MS chromatograms showing siderophore production in wild type UTI89 and strains with deletions in specific siderophore biosynthesis genes (Δ_entB_, Δ_iroB_, Δ_ybtS_, Δ_entB_/Δ_ybtS_). Chromatograms are shown at the retention times for enterobactin (m/z 670), salmochelin (m/z 1012), and ferric yersiniabactin (m/z 535). For each column, the vertical scale is a fixed fraction of the corresponding 13C internal standard peak height, allowing comparison between samples. B) Chrome azurol S (CAS) plate upon which UTI89 strains have been streaked for overnight growth. A yellow halo is produced around siderophore-secreting bacteria. In UTI89, a double mutant (Δ_entB_/Δ_ybtS_) is required to specifically abolish siderophore production.