Studies of a Ring-Cleaving Dioxygenase Illuminate the Role of Cholesterol Metabolism in the Pathogenesis of Mycobacterium tuberculosis (original) (raw)
Figure 5
Growth of a Δ_hsaC_ mutant of M. tuberculosis on cholesterol and in mice.
(A) Growth of H37Rv strains in minimal media containing 0.1% (v/v) glycerol, 0.8% (v/v) isopropanol (solvent control), 0.02% (w/v) cholesterol with 0.8% (v/v) isopropanol, or no added carbon source. The plotted values represent the means of triplicates, with error bars indicating standard deviation. (B) Accumulation of a colored metabolite during cholesterol utilization by the Δ_hsaC_ mutant. (C) Survival of SCID mice after intravenous infection with 105 CFU of wild-type H37Rv, the Δ_hsaC_ mutant or the complemented Δ_hsaC_ mutant, respectively (n = 10 mice per group).