De Novo Synthesis of VP16 Coordinates the Exit from HSV Latency In Vivo (original) (raw)
Figure 1
Replication of VP5p/VP16 mutants in vitro and in vivo.
(A) A diagram of the construction of the VP5p/VP16 mutants is shown. The VP16 promoter and 5′UTR sequences were replaced with those of another gene expressed with leaky late kinetics, the VP5 gene, as detailed in Methods. (B) RSC were infected with mutants VP5/VP16-1 and -3, the genomically restored isolate (VP5p/VP16-1R), and wild type HSV-1 strain 17Syn+ at an moi of 0.0004 pfu/cell. At the indicated times, 3 plates infected with each virus were harvested and assayed independently for virus content as detailed in Methods. (C,D) Mice were infected as detailed in Methods and, at the indicated times pi, tissues from three mice from each group were assayed for virus content. The grey shading in C and D indicates the regions employed to calculate the areas under the curves for the VP5p/VP16 mutants.