De Novo Synthesis of VP16 Coordinates the Exit from HSV Latency In Vivo (original) (raw)
Figure 2
Activation of the VP16 promoter in sensory neurons during acute infection.
(A) Mice were infected with 17VP16pLZ. At the indicated days pi, TG were removed and sequentially processed for the detection of b-gal activity (blue) and viral proteins (purple), as detailed in Methods. A photomicrograph of a focus of infection in a TG is shown. White arrows indicate neurons with b-gal activity with little or no detectable viral protein. Counts of neurons positive for only b-gal or b-gal plus viral protein are indicated below the micrograph. (B) Photomicrographs of viral plaques on RSC monolayers infected at low moi with 17-0pZ563gJ and 17VP16pLZ are shown. The monolayers were stained to detect b-gal and viral proteins. At regions at the edge of the plaques formed by 17-0pZ563gJ cells expressing b-gal (blue) with little or no evidence of viral protein expression were evident (arrow). Regions at the edge of plaques formed by 17VP16pLZ show cells staining positive for viral proteins (purple) with little or no staining for b-gal evident (arrow).