De Novo Synthesis of VP16 Coordinates the Exit from HSV Latency In Vivo (original) (raw)
Figure 4
Mutant in1814 establishes latency as efficiently as genomically wild type isolates.
Groups of mice were infected with strain 17Syn+, in1814, and the genomically rescued variant 1814R, as described in Methods. At 40 days pi, the ganglia of 3 mice per group were processed for single neuron PCR. Individual neurons were examined for the presence of the viral genome and the number of viral genomes present in positive neurons was determined using a quantitative PCR assay as detailed in Methods. (A) Shown is the percentage of neurons positive for the viral genome. The number of neurons positive for the viral genome over the number tested is shown in the histograms. (B) Each point on the scattergram represents the number of viral genomes present in an individual neuron. The horizontal bars are drawn at the mean value of genome copies per positive neuron.