Long-Distance Delivery of Bacterial Virulence Factors by Pseudomonas aeruginosa Outer Membrane Vesicles (original) (raw)
Figure 2
OMV deliver functional Cif virulence factor to airway epithelial cells, across a mucus layer.
(A) Purified OMV applied to the apical surface of airway epithelial cells reduce CFTR in the plasma membrane in a time-dependent manner, compared to the buffer control (Control), as measured by Western blot analysis. Overnight supernatant (PA14 O/N Sup), previously reported to decrease apical membrane CFTR, serves as a positive control [16]. (B) OMV purified from a P. aeruginosa Δ_cif_ mutant strain did not reduce apical membrane expression of CFTR, as measured by Western blot analysis. Black bar, wild-type OMV; striped bar, Δ_cif_ OMV. (C) Cif-containing OMV decrease apical membrane CFTR in airway epithelial cells (CFBE) and mucous-producing airway epithelial cells (Calu-3), as measured by Western blot analysis. Black bar, CFBE cells; striped bar, Calu-3 cells. Data are presented as mean+/−SEM. n = 3, * p<0.05 versus Control.