Retnla (Relmα/Fizz1) Suppresses Helminth-Induced Th2-Type Immunity (original) (raw)
Figure 8
Retnla suppresses S. mansoni egg-induced Th2 responses.
(A) Naïve and i.p. egg sensitized and challenged (secondary) WT (N = 5/time point) and Retnla−/− (N = 5/time point) mice were sacrificed on day 7 post i.v. egg-challenge. Right lobe RNA specimens were prepared individually for real-time PCR analysis of Retnla, Il4, Il5, and Il13. Gene expression (Mean±SEM) is expressed as the fold-increase over naïve WT controls after normalization to HPRT. Statistically significant differences and the corresponding p values are noted in the panels. (B) Lung leukocytes were isolated, separated, counted and cultured with PMA, Ionomycin and Brefeldin A for 3 hrs and analyzed for ex-vivo cytokine production. Total number of IL-4, IL-5, and IFN-γ producing CD4+ T cells in the lung leukocyte preparation are shown for individual mice. The number of IFN-γ producing non-CD4+ T cells was also included (bottom panel). (C) Magnitude of cytokine production by CD4+ T cells displayed as the geometric mean of fluorescence intensity. Statistically significant differences are shown. All experiments were repeated twice with similar results.