Cross-Talk between TLR4 and FcγReceptorIII (CD16) Pathways (original) (raw)
Figure 6
Expression levels of FcγRII/III, FcRγ-subunit, and C5aR on phagocytes from Wt and TLR4 mut mice.
(A) Summary of flow cytometry analyses of FcγRII/III expression on blood PMNs. (B,C) Original flow cytometry results for FcγRII/III expression on the surface of PMNs from Wt (B) or TLR4 mut (C) mice. (D,E) Analysis of the expression of FcγRIII (upper bands) and FcRγ-subunit (middle bands) in cell lysates [(D), PMNs; (E), macrophages] from Wt or TLR4 mut mice by Western blotting. The lower bands represent the analysis for GAPDH as loading controls. (F) Surface expression of C5aR protein on PMNs from Wt or TLR4 mut mice as assessed by flow cytometry. MFI, mean fluorescence intensity. Studies were done in three separate and independent experiments, with each sample run in duplicates.