Innate Immune Sensing of Modified Vaccinia Virus Ankara (MVA) Is Mediated by TLR2-TLR6, MDA-5 and the NALP3 Inflammasome (original) (raw)
Figure 4
TLR2, TLR6 and MyD88 are critical for IFNβ-independent chemokine production after MVA infection.
MIP-2 (A) and IFNβ (B) produced by wild-type, TLR1−/−, TLR2−/−, TLR4−/−, TLR6−/−, MyD88−/− and TRIF−/− bone marrow-derived macrophages infected with MVA (MOI 5 and 20) or stimulated with lipopolysaccharide (LPS, 100 ng/ml), Pam2CSK4 (P2CSK4, 10 µg/ml), Pam3CSK4 (P3CSK4, 10 µg/ml) for 24 h. Data are means±SD of triplicate samples from one experiment and are representative of 2 to 4 experiments.