The Glyceraldehyde-3-Phosphate Dehydrogenase and the Small GTPase Rab 2 Are Crucial for Brucella Replication (original) (raw)
Figure 1
Survival and replication of B. abortus inside BHK-21 cells.
(A) Fold increase of intracellular replication of B. abortus over 48 h within 3 different cell types: BHK-21 fibroblasts, BMDM and Raw 264.7 macrophages. Data are means±SD of three independent experiments. (B) Confocal microscopy micrographs of BHK-21 infected for 48 h with B. abortus expressing GFP. Fixed cells were stained with anti-calnexin antibody (red) to label the ER. (Scale bars: 10 µm). (C) Electron microscopy micrographs of thin sections of BHK-21 cells infected with B. abortus and fixed 48 h later. General view of an infected cell displaying several BCVs. Note that most BCVs contain more than one bacterium. (D) Enlarged view of a BCV containing several bacteria. (E) Enlarged view of part of the BCV shown in D. Note the ribosomes along the BCV membrane (arrows). (Scale bars: 0.5 µm).