The Glyceraldehyde-3-Phosphate Dehydrogenase and the Small GTPase Rab 2 Are Crucial for Brucella Replication (original) (raw)
Figure 3
GAPDH and Rab 2 localise to the BCV membrane.
(A) Western blotting of the enriched fraction of BCVs: A/Interface 50%–12%, B/Sucrose 50%, C/Sucrose step gradient. Immunoblotting with anti-GAPDH, anti-Rab 1 and anti-Rab 2 antibodies. (B) Confocal microscopy micrographs of isolated BCVs from the sucrose step gradient. B. abortus GFP (green) and Rab 2 staining (red). (Scale bars: 5 µm).