The Glyceraldehyde-3-Phosphate Dehydrogenase and the Small GTPase Rab 2 Are Crucial for Brucella Replication (original) (raw)
Figure 6
The inhibition of PKC ι/λ, COPI, Rab 2 and Enolase expression affects B. abortus replication at 48 h p.i.
(A) Western blotting of cellular extracts from cells transfected with PKC ι siRNA, COP B siRNA, Rab 2 A siRNA, Enolase siRNA or control siRNA-A. Immunoblotting with anti-PKC ι, -COP B, -Rab 2 A, -Enolase and anti-actin antibodies. (B) Fold increase of intracellular CFUs at 48 h p.i. of transfected Hela cells with PKC ι siRNA, COP B siRNA, Rab 2 A siRNA, Enolase siRNA or control siRNA-A and infected with B. abortus. Data are means±SD of three independent experiments.