Evidence for a “Wattle and Daub” Model of the Cyst Wall of Entamoeba (original) (raw)
Figure 6
Recombinant Jessie 3 lectins self-aggregate.
Fluorescence microscopy (A to D) and negative staining (E) of bacteria transformed with MBP-fusion-proteins expressing full-length Jessie3 (green in A) or the unique C-terminal unique domain of Jessie3 (green in B) agglutinate to form large clumps of bacteria. In addition, biofilms containing MBP-full length Jessie3 are formed (green in D and immunostained with gold in E). Antibodies to the unique C-terminal domain of Jessie 3 are conjugated to Alexafluor in (A, B, and D) or are detected with immunogold in (E). Antibodies to the N-terminal CBD of Jessie3 (green in C) show that MBP-fusion-proteins containing this domain do not self-aggregate on a macro-scale and do not agglutinate bacteria. Bar (A to D) is 2 microns. Bar (E) is 500 nm.