NOD2, RIP2 and IRF5 Play a Critical Role in the Type I Interferon Response to Mycobacterium tuberculosis (original) (raw)
Figure 4
Type I Interferon production upon Mtb infection is reduced in Rip2- and Nod2-deficient macrophages.
A, B, E, and F. BMDM derived from wt, rip2−/− and nod2−/− mice were infected with Mtb (MOI 10) for 4 h. RNA was harvested and IFNα, IFNβ, RANTES and TNFα mRNA levels were quantified using real time PCR. Gene expression is reported as copy number per 1,000 copies of β-actin. Samples were assayed in triplicate; error bars represent the standard deviation. The experiment shown is representative of at least three. Statistical evaluation was performed using an unpaired Student's t test. p-values>0.05 are reported as “n.s.” (i.e. not significant). C and D. BMDM derived from wt, rip2−/− and nod2−/− mice were infected with Mtb (MOI 10) for 18 h, the amount of IFNα and IFNβ released in the supernatant was quantified by ELISA. Samples were assayed in triplicate; error bars represent the standard deviation. N.D. indicates not detected, that is the actual value is below zero in standard curve.