Target Cell Cyclophilins Facilitate Human Papillomavirus Type 16 Infection (original) (raw)
Figure 3
Inhibition of CyP leads to noninfectious HPV16 internalization.
(A) HaCaT cells were infected with HPV16 pseudovirus in the absence or presence of NIM811. At 18 hpi cells were fixed and stained using conformation-dependent NmAb H16.56E or linear epitope-specific nonneutralizing 33L1-7. Cells were also stained for actin and DNA using AF488–labeled phalloidin (green) and Dapi (blue), respectively. Images were taken with a Leica DBMI6000 at 40× magnification. (B) HaCaT cells were transfected with indicated siRNA. At 48 hpTx, cells were harvested and reseeded for infection with HPV16 pseudovirions. At 18 hpi cells were fixed and stained using H16.56E (red) and CyPA or CyPB (green). Images were taken with a confocal Zeiss LSM 510 microscope at 63× magnification.