Enterovirus 71 3C Protease Cleaves a Novel Target CstF-64 and Inhibits Cellular Polyadenylation (original) (raw)
Figure 2
CstF-64 in 3Cpro-treated nuclear extract.
(A) CstF-64 proteins in nuclear extracts from SF268, RD and HeLa cells were detected using anti-CstF-64 antibodies. Untreated nuclear extracts (-) were loaded as controls. The amount of CstF64 in wild-type 3Cpro treated nuclear extracts (WT) and in mutant 3Cpro-treated nuclear extracts (C147S) were showen. A cleavage product of 55 kDa was indicated (*). PCNA (proliferatory cell nuclear antigen) detection was employed as the loading control. (B) CstF-64 protein in RD nuclear extract treated with various quantities of 3Cpro - 0.5 µg , 1 µg, 2 µg , 4 µg and 5 µg (lanes 3 to 7) and in the mutant 3C-treated nuclear extract (C147S) or untreated nuclear extract (-) were shown. (C) eIF4-GI in wild-type (WT) and mutant 2Apro (C110S) treated RD cell extracts were detected using specific antibody (lanes 1–3). CstF-64 in 2Apro or mutant 2Apro-treated nuclear extract was also detected (lanes 4–6).