Co-Regulation of NF-κB and Inflammasome-Mediated Inflammatory Responses by Myxoma Virus Pyrin Domain-Containing Protein M013 (original) (raw)
Figure 2
Activation of early inflammatory responses by vMyxM013-KO virus.
THP-1 cells were differentiated with PMA and infected with MYXV or vMyxM013-KO viruses, at MOI of 3 or treated with LPS (1 µg/ml) for indicated time points and harvested to prepare total cell extracts. Equal amounts of protein from uninfected, infected or LPS treated cells were analyzed by Western blot using IκBα (A and B) and phospho IκBα (pIκBα) (C) antibodies.