Nucleoporin 153 Arrests the Nuclear Import of Hepatitis B Virus Capsids in the Nuclear Basket (original) (raw)
Figure 3
Competition of P-rC, Imp β and Nup153 interactions.
A. Nup153 was preincubated with importin β and P-rC. The complex was precipitated by anti capsid antibody-coated beads and Nup153 was detected by Immune blot using mAb414. − negative control without P-rC, +: positive control in the absence of Imp β. 1–5. Different ratios of capsid-importin β. 1: 1∶33, 2: 1∶50, 3: 1∶100, 4: 1∶150, 5: 1∶300. B. Anti capsid antibody-coated beads were preloaded with P-rC. P-rC was then saturated with Nup153 followed by addition of different excesses of Imp β.−: and +: as in A. Molar ratio capsid-Imp β 1: 1∶2600, 2: 1∶4000, 3: 1∶5400, 4: 1∶6700. The figure shows that even an excess of Imp β cannot displace Nup153-bound capsids. After Nup153 attachment to the capsid even excessive amounts of Imp β cannot replace Nup153.