Nucleoporin 153 Arrests the Nuclear Import of Hepatitis B Virus Capsids in the Nuclear Basket (original) (raw)
Figure 4
Nuclear import of P-rC in nuclei of partially Nup153-silenced cells.
A. Immune blot of Nup153 in Nup153-silenced cells. Upper panel Nup153, lower panel: the house keeping gene Fibrillarin. The blot shows a significantly reduced Nup153 expression B. Nuclear import in digitonin-permeabilized cells. Left: control mock-transfected cells. Right: Nup153-silenced cells. Capsids and Nup153 are visualized by indirect immune fluorescence (see as well video in Supporting information). Consistent with the incomplete Nup153 knock down some P-rC entered the nucleus exclusively in Nup153 siRNA-treated cells.