A New Nuclear Function of the Entamoeba histolytica Glycolytic Enzyme Enolase: The Metabolic Regulation of Cytosine-5 Methyltransferase 2 (Dnmt2) Activity (original) (raw)
Figure 2
Enolase is present in the cytoplasmatic and nuclear fraction of E.histolytica.
A. Cytoplasmatic (C) and nuclear (N) protein fractions of E.histolytica HM-1∶MSS and pJST4-Ehmeth trophozoites were separated on 12% SDS-PAGE and analyzed by western blot with an anti HA antibody, an anti enolase antibody, an anti EhMLBP antibody or an anti Myosin II antibody. B. Cellular localization of Ehenolase in E.histolytica trophozoites. Ehenolase was detected by immunofluorescence microscopy using anti-enolase antibody. Ehenolase distribution is shown in red using a primary anti-enolase antibody and a secondary antibody conjugated with Cy3. Nuclei (blue) were stained by DAPI. Computer-assisted image overlay analysis of the signal given by enolase antibody and by DAPI, shows that Ehenolase is ubiquitously present in trophozoites including in the nucleus. C. Cytoplasmatic and nuclear protein fractions of E.histolytica HM-1∶MSS, trophozoites expressing a NLS-fused-scramble peptide (NLS-Con) (30) and trophozoites expressing a NLS-fused enolase (NLS-Eno) were separated on 12% SDS-PAGE and analyzed by western blot with an anti enolase antibody, an anti actin antibody or an anti EhMLBP antibody.