A New Nuclear Function of the Entamoeba histolytica Glycolytic Enzyme Enolase: The Metabolic Regulation of Cytosine-5 Methyltransferase 2 (Dnmt2) Activity (original) (raw)
Figure 5
Enolase inhibits Ehmeth and hDnmt2 functions.
A. The binding of γ−ATP labeled EhMRS2 DNA (EhMRS2*−0.33 µg) to Ehmeth or hDnmt2 was detected as a DNA-protein complex. No complex was observed between GST and GST-Enolase incubated with EhMRS2 DNA. Enolase inhibits the binding of Ehmeth and hDnmt2 to EhMRS2 DNA in a dose dependent manner. B. Effect of enolase on the Ehmeth (left panel) and hDnmt2 (right panel) tRNA methyltransferase activity. The results represent the mean and standard deviation of three independent experiments (Pvalue<0.05). U = one unit corresponds to 1 pmol of H3-Adomet incorporated/hour/nmol of enzyme.