Production of Extracellular Traps against Aspergillus fumigatus In Vitro and in Infected Lung Tissue Is Dependent on Invading Neutrophils and Influenced by Hydrophobin RodA (original) (raw)
Figure 6
NET formation in vivo is dependent on the presence of newly immigrating neutrophils.
(A) Mice were intratracheally injected with either swollen A. fumigatus conidia or PBS. 7 h after infection, the number of neutrophils in the bronchoalveolar lavage of these mice was measured by FACS. (B) Lys-EGFP mice were treated with the neutrophil depleting anti Gr-1 antibody RB6-8C5 24 h before infection with A. fumigatus. A lung slice of such a mouse analysed 10 h after infection shows almost no green cells (arrowhead in the magnification of the area boxed in green shows one of the very rare cells in this slice) and no NET-like structures (note only punctate red staining for DNA of nuclei) in areas of fungal masses (blue). (The area of the red square is magnified on the right) The image is representative for 3 animals that were analysed. (C) Quantification of NET formation in Gr-1- and mock-depleted mice infected with swollen conidia as well as untreated mice infected with resting conidia. Shown is a representative result of 3 independent experiments performed. For each condition 20 fungal clouds >20 µm were scored for the presence of NET structures. The 3 images are reference pictures for the type of structure scored with −/+/++.