Production of Extracellular Traps against Aspergillus fumigatus In Vitro and in Infected Lung Tissue Is Dependent on Invading Neutrophils and Influenced by Hydrophobin RodA (original) (raw)
Figure 8
Survival of resting and swollen conidia of A. fumigatus strains after co-incubation with neutrophils.
The number of CFU determined for conidia without co-incubation (T0) was set as 100% survival. CFUs of A. fumigatus conidia were determined as described in materials and methods. Survival of swollen (grey bars) and resting conidia (white bars) are depicted. (A) Survival of A. fumigatus strain ATCC46645 during co-incubation with neutrophils over time (T0, T60, T120 and T180). Asterisks indicate significant difference (*p<0.05 or **p<0.01) in survival in comparison to the time point T0 for each morphotype. (B) Survival of A. fumigatus strain ATCC46645 after co-incubation with neutrophils and in the presence of DNase I or DPI. Asterisks indicate significant difference (*p<0.05 or **p<0.01) in survival in comparison to the time point T180 after neutrophil co-incubation. The survival rate did not increase significantly by the addition of DNase I or the NAD(P)H-oxidase inhibitor DPI. Only the addition of cytochalasin D increased the survival of A. fumigatus conidia. (C) Analysis of the survival of the A. fumigatus strain pksP and its parental wild type strain ATCC46645 showed no significant differences in killing. Also the deletion mutant Δ_rodA_ and its parental wild type strain DAL revealed no difference in killing. The addition of the conidial hydrophobin RodA (0.07 µg [w/well]) did not influence the survival of the Aspergillus fumigatus mutant strain Δ_rodA_ and the wild type strain DAL during co-incubation with neutrophils.