Neisseria meningitidis Induces Brain Microvascular Endothelial Cell Detachment from the Matrix and Cleavage of Occludin: A Role for MMP-8 (original) (raw)
Figure 2
Effect of N. meningitidis on the tight junction proteins occludin, ZO-1 and claudin-1.
HBMEC were infected with N. meningitidis strain MC58 for indicated time points and cells were immunostained for (A) occludin, ZO-1 and (B) separately stained for claudin-1. Control, non-infected HBMEC. Control cells that have been stained for occludin showed the expected localizations. Cells infected with N. meningitidis strain MC58 showed dissociation of occludin (green fluorescence) from the cell membrane and a diffuse localization in the cytoplasm at 24 h p.i. Localization of ZO-1 (red fluorescence) and claudin-1 (magenta fluorescence) was not changed in the remaining cells.