Prolonged Antigen Presentation Is Required for Optimal CD8+ T Cell Responses against Malaria Liver Stage Parasites (original) (raw)
Figure 6
Prolonged antigen presentation is required for optimal CD8+ memory formation.
A and B. Naïve mice received 2×103 transgenic cells and were immunized on d0 with 5×104 irradiated P. yoelii sporozoites. On either d4 or d7 CD8+ T cells were purified from these mice and the equivalent of 5×104 transgenic cells were transferred to recipients that had received 5×104 irradiated P. yoelii sporozoites or 5×104 irradiated P. berghei sporozoites on d0. On d35 the size of the transgenic memory population was measured by FACs. A. Outline of the experiment. B. Size of the resulting memory populations after cells transfer on (i) d4, (ii) d7 (n = 5, mean ± SE, ** = P<0.01). C and D. BALB/c mice were immunized with 5×104 irradiated sporozoites and 2×103 d60 memory transgenic cells were transferred to mice at different time points after immunization. Ten days after transfer the transgenic cells were recovered and the number of divided cells was determined C. Outline of the experiment. D Number of divided cells recovered (n = 3, mean ± SE; a positive response is considered > upper 95% confidence interval of the response seen in naïve mice, data from one of two similar experiments).