Bordetella Adenylate Cyclase Toxin Mobilizes Its β2 Integrin Receptor into Lipid Rafts to Accomplish Translocation across Target Cell Membrane in Two Steps (original) (raw)
Figure 4
Mobilization of CyaA into lipid rafts depends on influx of extracellular Ca2+ ions into cells.
(A) J774A.1 cells were incubated at 37°C for 10 min with 1 nM CyaA and CyaA-E570K+E581P, in the presence or absence of 500 nM ionomycin , or of 1 µM thapsigargin, respectively. Cell lyzates were analyzed on sucrose density gradients as above. The blots are representative of four independent experiments. (B) Distribution of CyaA proteins in gradient fractions was quantified as in Fig. 1 and the values represent the mean ± S.D. of three independent experiments. An asterisk indicates a statistically significant difference (*, p<0.05; Student's t test).