Formation of Complexes at Plasmodesmata for Potyvirus Intercellular Movement Is Mediated by the Viral Protein P3N-PIPO (original) (raw)
Figure 5
Association of TuMV CP with CI in TuMV-infected N. benthamiana leaf cells.
(Panels I, II) TuMV CP tagged by YFP (YFP-CP) in the cytoplasm when expressed alone (panel I) or during virus infection (panel II). (Panel III) When coexpressed with the recombinant TuMV::6K-GFP infectious clone, some mRFP-CP is also present in proximity to the 6K-GFP-labeled replication complex (arrow). (Panel IV) TuMV YFP-CP attachment to PD-associated CI structures.(arrows; Inset) in the cell periphery in the presence of P3N-PIPO during virus infection. All images are taken 48 hrs post-agroinfiltration. Chl, chloroplasts. Bars, 8 _µ_m.