Balanced Nuclear and Cytoplasmic Activities of EDS1 Are Required for a Complete Plant Innate Immune Response (original) (raw)
Figure 3
Quantitative transcript profiling of EDS1-dependent genes in response to virulent and avirulent Pst DC3000 bacteria.
Four-week-old plants were spray-inoculated with 10 mM MgCl2 (Mock, white bars), Pst DC3000 (grey bars) or Pst DC3000 AvrRps4 (black bars) and leaf samples collected at 0, 1, 3, 8 and 24 h post inoculation. Bars represent means and standard deviation from three biological replicates. Transcript levels were determined by qRT-PCR and normalized using the internal control UBIQUITIN. (A) Relative transcript levels of genes displaying EDS1-dependent induction after infection with either bacterial strain. (B) Relative transcript levels of genes displaying EDS1-dependent repression during RPS4-mediated resistance with bars representing the fold change in Pst DC3000 AvrRps4-treated samples compared to mock-treated samples. * indicates significant differences between expression at the indicated h post inoculation and 0 h in each genotype and ° indicates significant differences between Col-0 and eds1-2 at the indicated time point (t-test, p-value <0.01). Slower transcriptional changes in response to Pst DC3000 AvrRps4 compared with previous analysis (Table S2, [5]) are probably due to the use of spray inoculation instead of leaf infiltration for bacterial challenge.