PB1-F2 Proteins from H5N1 and 20th Century Pandemic Influenza Viruses Cause Immunopathology (original) (raw)
Figure 2
PB1-F2 causes cell death.
Induction of cell death was determined in human epithelial A549 and mouse macrophage RAW264.7 cell lines by detection of Annexin+PI+ events after A & B) exposure to a panel of 27-mer peptides derived from the C-terminal region of the PB1-F2 protein (see Figure 1 for definition), an N-terminal 27-mer peptide derived from PR8 (final concentration 50 µM for each peptide), or no peptide for 1 hour; and infection with a panel of recombinant virus (see methods for definitions of viruses; the H3N2 PB1 is from A/Wuhan/359/95) at a multiplicity of infection of 1.0 at C) 12 hours and D) 8 hours post-infection. An asterisk (*) indicates a significant difference compared to A) all other peptides and controls, B) all peptides and controls except PR8 and 1918, C &D) all viruses with a PB1 gene segment derived from an H1N1 virus and the no virus control by ANOVA (p<0.05).