Early Severe Inflammatory Responses to Uropathogenic E. coli Predispose to Chronic and Recurrent Urinary Tract Infection (original) (raw)
Figure 5
C3H/HeN mice that develop chronic cystitis have severe bladder inflammation at 24 hpi.
Individual C3H/HeN mice were infected with either 107 or 108 cfu of UTI89 and bladders and sera collected at 24 hpi in two independent experiments. Data shown are from one representative experiment. A, Paraffin-embedded, fixed bladder sections were stained with hematoxylin & eosin and examined by light microscopy. Sections were scored from 0–5 to quantify the severity of inflammatory lesions as explained in the Materials and Methods; horizontal bars indicate median values. B, Examples are shown of the disparate response to UPEC infection in two individual mice from the same cage, infected on the same day with the same inoculum (107 cfu UTI89). C–D, Higher magnification images of the two bladders shown in panel B. Inflammatory scores were 3 and 5 for panels C and D, respectively. Bars approximate 50 µm in length. E, Scatter plot analysis of serum IL-5, IL-6, G-CSF, and KC values, as labeled, versus the inflammatory scores of the bladders from the same individual mice. Significance (P) and strength (ρ) of correlations shown were determined by Spearman's rank correlation test.