Early Severe Inflammatory Responses to Uropathogenic E. coli Predispose to Chronic and Recurrent Urinary Tract Infection (original) (raw)
Figure 6
C3H background severe combined immunodeficient mice have muted acute inflammatory responses to UPEC infection and are resistant to chronic cystitis.
C3H/HeSnJ (closed circles) or C3SnSmn.CB17-Prkcscid/J (C3H_scid_, open circles) mice were infected with 108 cfu of UTI89 KanR or UTI89. A, Urine and tissue titers were assayed at 4 wpi; solid lines connect the different urine and tissue titers from the same mouse. B, PMN scores of the urine sediments were determined at 24 hpi. The data shown in panels A and B were compiled from four independent experiments. C, Sera were collected for cytokine analysis at 24 hpi. Data are representative of two independent experiments. All statistics in panels B–C are the results of the Mann-Whitney U test: **, P<0.01, ***, P<0.001 and ns, not significant, horizontal bars indicate median values. D–E, Representative paraffin-embedded, fixed bladder sections from D, C3H/HeSnJ, and E, C3H_scid_ mice, at 24 hpi were stained with hematoxylin & eosin and examined by light microscopy. Asterisks indicate areas of submucosal edema, arrows indicate apparent IBCs, and bars approximate 50 µm in length.