The Coxiella burnetii Dot/Icm System Delivers a Unique Repertoire of Type IV Effectors into Host Cells and Is Required for Intracellular Replication (original) (raw)
Figure 1
Dot/Icm-dependent translocation of C. burnetii proteins by L. pneumophila.
CHO-FcγRII cells were infected with Dot/Icm-sufficient LP01 strain of L. pneumophila (black) or the isogenic Δ_dotA_ mutant (grey) expressing Cya fusions to the indicated C. burnetii proteins. (A) Fusions to Cya of the indicated full-length derivatives of C. burnetii NM proteins identified in the genetic screen were tested for translocation (B). Fusions to Cya of the indicated full-length derivatives of C. burnetii NM proteins identified based on homology or proximity to proteins identified in the screen were tested for translocation. Cya indicates empty vector control. Cya-RalF was used as a positive control. After 1 h, host cells were lysed and cAMP was extracted. Total cAMP levels resulting from translocation of protein fusions were quantified using an enzyme-immunoassay system, and are shown in fmol. Results represent average values +/− SD of experiments performed in triplicate.