Drosophila melanogaster as an Animal Model for the Study of Pseudomonas aeruginosa Biofilm Infections In Vivo (original) (raw)

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Figure 3

Visualization and staining of in vivo microcolonies in the Drosophila crop.

(A) DAPI-stained bacterial cells (100X) and (B) digitally zoomed images (4.4X) of DAPI stained microcolonies in the crop, demonstrating a honeycomb-like structure (white arrow). Scale bars in A indicate 200 µM. Scale bars in B indicate 45.4 µM. At least three infected crops were examined from three separate infections and representative images are shown. Honeycomb-like structures were visualized in 2 out of every three PAO1-infected crops examined.

Figure 3

doi: https://doi.org/10.1371/journal.ppat.1002299.g003