Drosophila melanogaster as an Animal Model for the Study of Pseudomonas aeruginosa Biofilm Infections In Vivo (original) (raw)
Figure 6
In vivo localization and antibiotic resistance profiling of biofilm and non-biofilm infections.
(A) Localization of bacteria in the fly 5 days postinfection. The GI tract including the crop, was dissected out, crushed and plated on PIA agar to determine CFU per GI tract/fly. The remainder of the fly body, including the head, was crushed separately and plated on PIA to determine CFU/rest of body per fly. (B) The number of CFU recovered from Drosophila hemolymph 2- and 5-days postinfection with PAO1, PAZH13 or pelB::lux. Two biological replicate experiments were performed, each containing 20 Drosophila, and values represented are mean +/−SEM. (C) Antibiotic resistance profiling of biofilm and non-biofilm infections. Increase in antibiotic resistance, as measured by zone of inhibition in disk diffusion assay, in P. aeruginosa strains recovered for Drosophila after oral infection relative to planktonic cultures. Antibiotic concentration indicated in µg/ml. Two biological replicate experiments were performed in triplicate and mean +/− SEM is shown. * p<0.05, ** p<0.01, ***p<0.001.