Drosophila melanogaster as an Animal Model for the Study of Pseudomonas aeruginosa Biofilm Infections In Vivo (original) (raw)
Figure 8
Biofilm infections induce antimicrobial peptide gene expression in Drosophila.
Real time RT-PCR analysis of (A) cecropin A1 (B) diptericin and (C) drosomycin following oral infection with PAO1, pelB::lux, PAZH13 or following oral co-infection with a 1∶1 ratio of PAO1-PAO1p16S_lux_, PAO1p16S_lux_-pelB::lux or PAO1p16S_lux_-PAZH13. For co-infection experiments (last 3 bars) the strains used for each infection are listed, separated by a hyphen. The levels of AMP gene expression was represented as fold change relative to uninfected flies. Values are mean +/− SEM from triplicate qPCR experiments on RNA isolated from two independent Drosophila infections. a, significant fold change (p<0.05, ANOVA) relative to uninfected flies; b, significant fold change (p<0.05, ANOVA) relative to PAO1-infected flies. (D) Kaplan-Meier survival curves of Drosophila following oral co-infection with a 1∶1 ratio of PAO1-PAO1p16S_lux_, PAO1p16S_lux_-pelB::lux, PAO1p16S_lux_-PAZH13 and relevant controls. Experiments were performed at least 3 times each with a minimum of 80 flies and representative curves (mean +/− standard deviated) are shown. a, significant difference (p<0.05, ANOVA) relative to PAO1-PAO1p16S_lux_-infected flies (green); b, significant difference (p<0.05, ANOVA) relative to _pelB::lux_-infected flies.