Two Distinct Coagulase-Dependent Barriers Protect Staphylococcus aureus from Neutrophils in a Three Dimensional in vitro Infection Model (original) (raw)
Figure 6
Schematical drawing of the setup.
Staphylococci are grown in 3D-CoG/Fib in wells for 16 h in the absence of neutrophils. Then the medium is removed, stainings (e. g. Sytox Blue) can be applied and the 3D-CoG is overlaid with a 300 µm thin native spleen slice. Neutrophils migrate from the tissue slice into the 3D-CoG where they interact with staphylococci. The bottom of the well is suitable for confocal microscopy.