HIV-1 Capsid-Cyclophilin Interactions Determine Nuclear Import Pathway, Integration Targeting and Replication Efficiency (original) (raw)
Figure 2
Nup358Cyp evolved under positive selection pressure.
A, Codon positions under positive (dN>dS) and negative (dN<dS) selection along the Nup358Cyp gene sequence, as indicated by the Bayesian probability that a particular site is under selective pressure. A Bayes factor >50 is considered strong evidence for the favored model. B, Maximum likelihood phylogenetic tree of cyclophilins A, B, C, D, E, F, G, H and Nup358Cyp from Rhesus macaque (M. mulatta), Human (H. sapiens), Chimpanzee (P. troglodytes), Dog (C. familiaris), Cow (B. taurus), Horse (E. caballus), Marmoset (C. jacchus), Mouse (M. musculus), Rat (R. norvegicus), Opossum (M. domestica), Zebrafish (D. rerio), Orangutan (P. abelii) and Rabbit (O. cuniculus). Branch lengths and bootstrap supports are shown. C, Maximum likelihood phylogenetic tree demonstrating the simplest model of evolution of residue 61 in Nup358Cyp sequences. D, Structure of CypA bound to CANTD with CypA key residues R55 and M61 as well as HIV-1 CA P90 highlighted. (PDB:1FGL) E, NL4.3 HIV-1 GFP vector titer on CRFK cells expressing empty vector (EV), TRIMCypA, wild type TRIMNup358, or TRIMNup358 mutant V61M. Protein levels measured by western blot detecting the HA tag with β-Actin as loading control are shown. The data are representative of three independent experiments (mean and SD, n = 3).