Eurasian-Origin Gene Segments Contribute to the Transmissibility, Aerosol Release, and Morphology of the 2009 Pandemic H1N1 Influenza Virus (original) (raw)
Figure 5
Ferrets infected with the recombinant pH1N1 virus release submicron particles containing influenza virus.
Q-PCR for influenza A M gene on RNA extracted from the filter of the cyclone-based air samplers. Air was collected for one hour on the outside of the infected ferret cage, each bar represents the amount of particles released by a single ferret infected with Rec pH1N1 (A), Eurasian (B), TRS (C), or 6∶2 reassortant (D). Absolute RNA was quantitated using a standard curve of in vitro transcribed influenza M gene RNA. Inf stands for infected ferret.