The Membrane Fusion Step of Vaccinia Virus Entry Is Cooperatively Mediated by Multiple Viral Proteins and Host Cell Components (original) (raw)
Figure 6
Entry of virions lacking H2 or A28 protein determined by transmission electron microscopy.
Purified MVs (350 PFU per cell or equivalent number of particles) possessing (+) or lacking (-) H2 or A28 protein as indicated were spinoculated onto pre-chilled HeLa cell monolayers at 4°C for 60 min. Virus-bound cells were then incubated for either 30, 60 or 180 min at 37°C, fixed and processed for electron microscopy. For each infection, a total of 90 randomly-selected cell sections were inspected and the number of full fusion events (A and C) and free viral cores in the cytosol (B and D) were determined as described in the legend to Figure 4.