Functional Amyloids Composed of Phenol Soluble Modulins Stabilize Staphylococcus aureus Biofilms (original) (raw)
Figure 7
An αβPSM mutant forms biofilms susceptible to disassembly by matrix degrading enzymes and mechanical stress.
Confocal micrographs of Δ_αβpsm_ mutant (A) (strain BB2388) versus complemented mutant expressing α and βpsm operons in trans (B) (strain BB2408) flow cell biofilms grown for 30 hours prior to proteinase K, dispersin B, and DNaseI exposure (at 0.2 µg/mL each). Images are representative of three separate experiments and each side of a grid square represents 20 µm. (C) Analysis of biofilm development at the air-liquid interface of test tube cultures in PNG media after vortexing. Graph shows quantification of biofilm biomass (OD A595) and images below show stained test tube biofilms. * P<0.005 compared to wildtype.