Global Metabolic Profiling of Infection by an Oncogenic Virus: KSHV Induces and Requires Lipogenesis for Survival of Latent Infection (original) (raw)
Figure 3
Fatty acid synthesis inhibitors selectively induce cell death in KSHV latently infected endothelial cells.
A) Schematic of lipogenesis pathway and where specific inhibitors block the pathway. At 48 hpi, mock- and KSHV-infected TIME cells were treated with B) 0, 10, or 20 µg/mL of TOFA and incubated for an additional 48 hours or C) 0, 4 or 8 µg/mL C75 and incubated for an additional 24 hours. Cell death rates were then determined by a trypan blue exclusion assay. Cell death rate (%) is # of dead cells/# of total cells. 48 hpi, TIME cells (D & E) or 1° hDMVECs (F & G) were treated with 0 µg/mL or 20 µg/mL TOFA for 36 hours (C & E) or 0 µg/mL or 4 µg/mL C75 for 24 hours (D & F). Cells were then treated with Image-It Dead Green viability stain and visualized on an inverted fluorescent microscope.