Complexes of Vesicular Stomatitis Virus Matrix Protein with Host Rae1 and Nup98 Involved in Inhibition of Host Transcription (original) (raw)
Figure 3
Effects of silencing the expression of Rae1 and Nup98 on interaction with M protein.
(A) HeLa cells were transfected with Rae1 siRNA or with non-targeting (NT) siRNA. 48 hours post-transfection, lysates from Rae1 siRNA cells or NT siRNA cells were incubated with GST-M protein (M) or GST (G) on glutathione beads for 2 hours at 4 C. Cell lysates and bound fractions were analyzed by immunoblots probed for Rae1 or Nup98. (B) Cells were transfected with Nup98 siRNA or with non-targeting (NT) siRNA and analyzed as in (A).