Identification of a Novel Splice Variant Form of the Influenza A Virus M2 Ion Channel with an Antigenically Distinct Ectodomain (original) (raw)
Figure 2
Pseudoreversion of an M2-null virus.
(A). Plaque and HA titre plotted before (passage 0) and after each step of 6 serial passage experiments as a fraction of the corresponding mean values obtained from two independent stocks of WT virus passaged in parallel. (B) Average plaque size in MDCK cells of the indicated viruses before and after (P6) serial passage. Values are the mean + SEM of 15–92 plaques normalized to the average WT value from each experiment. *** = p<0.001 compared to WT. (C). Segment 7 polypeptide expression. Lysates from cells infected with the indicated viruses were analysed by SDS-PAGE and western blotting as labeled.