Attenuated Salmonella Typhimurium Lacking the Pathogenicity Island-2 Type 3 Secretion System Grow to High Bacterial Numbers inside Phagocytes in Mice (original) (raw)
Figure 2
SPI-2 T3SS mutants are present in far fewer infected foci per organ than the wild-type.
(A to C) Representative fluorescence micrographs of Salmonella within phagocytes in infected livers of C57BL/6 mice at 72 h p.i.. (A) S12023; (B) S12023 sseB; (C) S12023 sseB(psseB) [CD18+ cells (red), Salmonella (green), nucleic acid is indicated by DAPI (blue), scale bars, 75 µm]. (D and E) Box and whisker plots showing the median, interquartile range and maximum and minimum number of infected cells per field-of-view in spleens for (D) S12023, S12023 sseB and S12023 sseB p(sseB) at 72 h p.i., obtained from 100 random fields from 4 mice for S12023 and S12023 sseB(psseB) infected tissue and 200 random fields from 7 mice for S12023 sseB infected tissue, and (E) S12023 sseB at 0.5 and 72 h p.i., obtained from 100 random fields from 3 mice for S12023 sseB infected tissue at 0.5 h p.i., and 200 random fields from 7 mice for S12023 sseB infected tissue at 72 h p.i.