Bifurcation of Arabidopsis NLR Immune Signaling via Ca2+-Dependent Protein Kinases (original) (raw)
Figure 7
The compromised immune responses in wrky mutants.
(A) The bacterial growth in wrky8 and wrky48 mutant plants. Plant leaves were hand-inoculated with Pst avrRpm1 or avrRpt2 at 5×105 cfu/ml. The bacterial growth was measured 4 dpi. The data are shown as mean ± SE of three repeats, and the asterisk (*) indicates a significant difference with p<0.05 when compared with data from WT plants. (B) Effector-induced WRKY46 expression was reduced in wrky mutant plants. WRKY46 expression was detected in plants 6 hr after hand-inoculation with bacteria at 1×107 cfu/ml. The expression of WRKY46 was normalized to the expression of UBQ10. The data are shown as the mean ± SE from three independent biological replicates. * indicates a significant difference with p<0.05 when compared with data from WT plants. (C) Effector-induced SID2 expression was reduced in wrky mutant plants. (D) A model of bifurcate NLR immune signaling via specific and overlapping CPKs. TTSS: type III secretion system. The above experiments were repeated three to four times with similar results.