Nucleoporin NUP153 Phenylalanine-Glycine Motifs Engage a Common Binding Pocket within the HIV-1 Capsid Protein to Mediate Lentiviral Infectivity (original) (raw)
Figure 9
PF74 counteracts HIV-1 similarly in the face of Trim-NUP153C restriction or NUP153 knockdown.
Mock transduced and Trim-NUP153C expressing (A) or non-targeting control and NUP153 knockdown (B) HOS cells were infected with equal RT-cpm of denoted viruses in the presence of various PF74 concentrations. Results are shown as infectivity normalized to vehicle only control cells (top), or vehicle only infection for each cell type (bottom) to calculate EC90 values. Dashed lines represent Trim-NUP153C or NUP153 knockdown results in panels A and B, respectively. Results are an average of at least 3 experiments, with error bars denoting standard error. Calculated EC90 values are displayed with standard error.