Type I and Type III Interferons Drive Redundant Amplification Loops to Induce a Transcriptional Signature in Influenza-Infected Airway Epithelia (original) (raw)
Figure 5
Type I and III are redundant in the MTEC system.
(A) Total RNA from mock and PR8 infected cells was analysed using Affymetrix Mouse Genome 430 2.0 microarrays at 24 hpi. Supervised analysis was performed using statistical filtering (≥4-fold change relative to mock infected wild-type in at least one treatment group; 2-way ANOVA, p<0.01, Benjamini-Hochberg multiple test correction). (A) Heat map of the upregulated genes. (B) Quantitative RT-PCR analysis of RNA samples extracted from mock and PR8 infected wild-type and IFNAR1−/−IL-28Rα−/− double knock-out MTEC at 24 and 48 hpi. All transcripts were first normalized to HPRT levels and then expressed as fold induction relative to the mean of mock infected controls, +/− SEM. (C) Wild-type and IFNAR1−/−IL-28Rα−/− double knock-out MTEC were infected and RNA samples collected at the indicated time points. Viral replication was measured by qRT-PCR on the Influenza A Matrix gene and expressed as copy number +/− SEM. Asterisks indicate differences that are statistically significant (unpaired t test; *, P<0.05).